Product Description

Cytokeratin 7 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90386-50, BS90386-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08729(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. Cytokeratin 7 (also known as sarcolectin) agglutinates normal and transformed cells with a high affinity for simple sugars. Cytokeratin 7 also inhibits the synthesis of interferon-dependent secondary proteins thus reversing the antiviral effect of interferon induction and restoring cells to their status ad primum. In normal and transformed cells, Cytokeratin 7 localizes to the membrane.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cytokeratin 7 polyclonal antibody detects endogenous levels of Cytokeratin 7 protein.

Bioworld Molecular Weight: 51 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cytokeratin 7 on different lysates using anti-Cytokeratin 7 antibody at 1/1,000 dilution.
Positive control:
Lane 1: A549
Lane 2: HepG2
Lane 3: SK-BR-3
Lane 4: SW1990, ICC staining Cytokeratin 7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CK-7, K7, KRT7, CK 7, CK-7, CK7, Cytokeratin 7, Cytokeratin-7, D15Wsu77e, K2C7, K2C7, K7, Keratin 7, Keratin 7, type II, Keratin type II cytoskeletal 7, Keratin, 55K type II cytoskeletal, Keratin, simple epithelial, Keratin, simple epithelial type I, K7, Keratin, type II cytoskeletal 7, Keratin-7, Krt2-7, KRT7, MGC11625, MGC129731, MGC3625, Sarcolectin, SCL, Type II mesothelial keratin K7, Type-II keratin Kb7,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified