Cytokeratin 8 Monoclonal Antibody-MB65916

Cytokeratin 8 Monoclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: MB65916-50, MB65916-100

Sizes: 50µl, 100µl

Swiss-Prot: P05787

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of Cytokeratin 8 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cytokeratin 8 expression in Hela (A), mouse brain (B) whole cell lysates., Immunohistochemical analysis of Cytokeratin 8 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 54 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: CYK8; Keratin type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Cytokeratin 8. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified