D-Lactic Acid/Lactate (LA) Colorimetric Assay Kit - E-BC-K002-M

D-Lactic Acid/Lactate (LA) Colorimetric Assay Kit

Sizes: 48T, 96T, 500 Assays

Catalogue Numbers: E-BC-K002-M-48, E-BC-K002-M-96, E-BC-K002-M-500

Citations, Manuals and MSDS Available upon request.

Target Synonym: D-LA

Research Areas: Glycolysis and Lipid Metabolism

Result Type: Quantitative

Detection Instrument: Microplate reader

Tested Sample Types: Serum, plasma, animal tissue, saliva sample

Assay Time: 20 min

Detection Type: Colorimetric method

Sensitivity: 0.06 mmol/L

Detection Range: 0.06-8.0 mmol/L

Test Principle: Using NAD+ as H+ receptor, D-lactate dehydrogenase (LDH) catalyzes the reaction of D-lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of D-lactic acid can be calculated.#N/A

Inter CV: 7.7

Intra CV: 3.8

Storage: This product can be stored at 2~8°C for 12 months with shading light.

Shipping: Ice bag