Product Description

DAG1 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS70524-50, BS70524-100

Swiss-Prot: Q14118

Host: Rabbit

DAG1 Polyclonal Antibody-BS70524

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:1000 | IF/ICC,1:20 - 1:100 | IP,1:50 - 1:200

Background: This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 43kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using DAG1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s., Immunoprecipitation analysis of 300ug extracts of Hela cells using 3ug DAG1 antibody. Western blot was performed from the immunoprecipitate using DAG1 antibody at a dilution of 1:1000.

Alternative Name: 156DAG; A3a; AGRNR; DAG; MDDGA9; MDDGC7; MDDGC9; DAG1

Immunogen: Recombinant fusion protein of human DAG1(NP_004384.4).

Modification: Unmodified

Conjugate: Unconjugated