Product Description

DAP-5 (N65) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2414-50, BS2414-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P78344

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: Death-associated protein 5 (DAP-5) (also known as p97 and NAT1) is a member of the eukaryotic translation initiation factor 4G (elF4G) family. DAP-5 is ubiquitously expressed and is highly conserved among species. In response to activated FAS or p53, caspase cleaves DAP-5 at position 790 to yield a C-terminal truncated protein which is capable of forming complexes with eIF4A and eIF3. DAP-5 has homology to the carboxy-terminal portion of eIF4G, but lacks the N-terminal region of eIF4G, which is responsible for association with the CAP binding protein eIF4E. By forming translationally inactive complexes with eIF4A and eIF3, but not with eIF4E, DAP-5 functions as a general repressor of translation. During apotosis, the caspase-activated DAP-5 can mediate CAP-independent translation at least from its own internal ribosome entry site, thus resulting in a positive feedback loop responsible for the continuous translation of DAP-5. DAP-5 is also required for cellular differentiation, as it controls specific gene expression pathways.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DAP-5 (N65) polyclonal antibody detects endogenous levels of DAP-5 protein.

Bioworld Molecular Weight: ~ 90 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DAP-5 (N65) polyclonal antibody at 1:500 dilution
Lane 1: HepG2 cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: H9C2 cell lysate, Immunohistochemistry (IHC) analyzes of DAP-5 (N65) pAb in paraffin-embedded human breast carcinoma tissue at 1:5000. cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of DAP-5 (N65) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:5000. cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of DAP-5 (N65) pAb in paraffin-embedded human stomach carcinoma tissue at 1:5000. cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Eukaryotic translation initiation factor 4 gamma 2; eIF-4-gamma 2; eIF-4G 2; eIF4G 2; Death-associated protein 5; DAP-5; p97; EIF4G2; DAP5; OK/SW-cl.75

Immunogen: Synthetic peptide, corresponding to amino acids 35-80 of Human DAP-5.

Conjugate: Unconjugated

Modification: Unmodified