Product Description

DARPP-32 (phospho-T75) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4058-50, BS4058-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Dopaminergic signaling pathways, which are essential for mμltiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson’s disease and drug abuse. DARPP-32 (for dopamine and adenosine 3’, 5’-monophosphate-regμlated phosphoprotein of 32 kDa) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors resμlts in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimμlate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Protein phosphatase 1 regμlatory subunit 1B, DARPP-32, Dopamine- and cAMP-regμlated neuronal phosphoprotein, PPP1R1B, DARPP32

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human DARPP-32 around the phosphorylation site of Threonine 75.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-DARPP-32 (T75) polyclonal antibody detects endogenous levels of DARPP-32 protein only when phosphorylated at Thr75

Molecular Weight: ~ 32 kDa

Pathway: Neuronal and Glial Cell Marker Atlas, Dopamine Signaling in Parkinson's Disease Plathway,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9UD71

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only