DBC1 (3G4) Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB0057-50, MB0057-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q8N163
Host: Mouse IgG1
Reactivity: Human, Mouse, Rat, Monkey
Applications: WB, ICC IP,
All Applications: WB: 1:500~1000
ICC: 1:50~200
Background: Core component of the DBIRD complex,a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing:the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions.Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis.Inhibits SUV39H1 methyltransferase activity.As part of a histone H3-specific methyltransferase complex may mediate ligand-dependent transcriptional activation by nuclear hormone receptors.
Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of DBC1 and does not cross-react with related proteins
Bioworld Molecular Weight: Predicted band size: 103KDa
Observed band size: 120KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot detection of DBC1 in HeLa,A549,Jurkat,C6,3T3 and COS7 cell lysates using DBC1 mouse mAb (1:500 diluted)., Immunocytochemistry of HeLa cells using anti-DBC1 mouse mAb diluted 1:200., Immunoprecipitation analysis of Hela cell lysates using DBC1 mouse mAb.
Alternative Name: DBC1; DBC-1; NET35; p30DBC; p30 DBC; DBC.1; DBIRD complex subunit; KIAA1967
Immunogen: Synthetic peptide, corresponding to the N-terminus of Human KIAA1967.
Conjugate: Unconjugated
Modification: Unmodified