Product Description

DBH Polyclonal Antibody

Catalogue Numbers: BS5695-50, BS5695-100

Sizes: 50µl, 100µl

Alternative Name: Dopamine beta-hydroxylase; Dopamine beta-monooxygenase; Soluble dopamine beta-hydroxylase

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P09172

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Dopamine β-hydroxylase (DBH) catalyzes the conversion of dopamine to noradrenaline in the biosynthesis of catecholamines. DBH is selectively expressed in noradrenergic and adrenergic neurons, as well as in neuroendocrine cells, and it serves as a specific protein marker for noradrenergic processes. The active form of DBH is a homotetramer, which is found in the lumen of synaptic vesicles of corresponding nerve cells, where it localizes to both the membrane and cytosol. DBH is induced by nerve growth factor and insulin growth factor-1 and is regulated by intracellular second messengers protein kinase A, cyclic AMP, diacyl glycerol and Ca2+. Expression of DBH is transcriptionally mediated by Sp1, CREB and AP-1 proteins including c-Fos, c-Jun and JunD.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DBH polyclonal antibody detects endogenous levels of DBH protein.

Bioworld Molecular Weight: ~ 69, 78, 84 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DBH polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: NIH-3T3 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 445-490 of Human DBH.

Conjugate: Unconjugated

Modification: Unmodified