Product Description

DBI Polyclonal Antibody

Catalogue Numbers: BS60861-50, BS60861-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07108

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Long chain acyl-CoA esters (LCAs) act as both substrates and intermediates in metabolism, and as regulators of various intracellular functions. Acyl-CoA binding protein (ACBP) specifically binds to LCA with high affinity and regulates its availability. ACBP is structurally and functionally conserved among a diverse group of organisms, including human, rat, frog, insect, plant and yeast. DBI, the gene encoding human ACBP, which maps to chromosome 2, is highly expressed in liver, soleus muscle and heart. The ACBP protein is also abundant in cells with a high level of lipogenesis and de novo fatty acid synthesis. Expression of ACBP is significantly induced during adipocyte differentiation. DBI is a target gene for proliferator-activated receptor (PPAR) γ, and is directly activated by PPARγ/RXR&aipha; and PPAR&aipha;/RXR&aipha;, but not by PPARδ/ RXR&aipha;. In addition to acyl-CoA binding and transport, ACBP is also implicated in g-aminobutyric acid type A receptor binding, steroidogenesis and peptide hormone release.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DBI polyclonal antibody detects endogenous levels of DBI protein.

Extra Notes: Western blot (WB) analysis of DBI pAb at 1:1000 dilution
Lane1:PC12 whole cell lysate(40ug)
Lane2:CT-26 whole cell lysate(40ug)
Lane3:THP-1 whole cell lysate(40ug)
Lane4:The liver tissue lysate of Mouse(40ug)
Lane5:The heart tissue lysate of Rat(40ug)
Lane6:MCF-7 whole cell lysate(40ug)
Lane7:HepG2 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 10 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Acyl-CoA-binding protein; ACBP; Diazepam-binding inhibitor; DBI; Endozepine; EP; DBI

Immunogen: A synthetic peptide corresponding to residues in Human DBI.

Conjugate: Unconjugated

Modification: Unmodified