Product Description

DDB1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0133-50, MB0133-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16531

Host: Mouse

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB

All Applications: WB: 1:1000

Background: The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit.This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage.Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However,it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit.In addition,Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q,but no sequence alternations of this gene are demonstrated in Best disease patients.The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of DDB1 and does not cross-react with related proteins

Bioworld Molecular Weight: Predicted band size:127KDa
Observed band size:127KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western BlotDetection of DDB1 antibody in Hela, 3T3, C6, COS7, 293T and K562 cell lysates using DDB1 antibody (1:1000 diluted).

Alternative Name: DNA damage-binding protein 1; DDB p127 subunit; DNA damage-binding protein a; DDBa; Damage-specific DNA-binding protein 1; HBV X-associated protein 1; XAP-1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; XPE-binding factor; XPE-BF; Xeroderma pigmentosum group E-complementing protein; XPCe; DDB1; XAP1

Immunogen: Recombinant full length Human DDB1.

Conjugate: Unconjugated

Modification: Unmodified