DDB1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90398-50, BS90398-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q16531(Human) Q3U1J4(Mouse) Q9ESW0(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP,
All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
IP:1:10-1:50
Background: Damaged DNA binding protein (DDB) is a heterodimer composed of two subunits, p127 and p48, which are designated DDB1 and DDB2, respectively. The DDB heterodimer is involved in repairing DNA damaged by ultraviolet light. Specifically, DDB, also designated UV-damaged DNA binding protein (UV-DDB), xeroderma pigmentosum group E binding factor (XPE-BF) and hepatitis B virus X-associated protein 1 (XAP-1), binds to damaged cyclobutane pyrimidine dimers (CPDs). Mutations in the DDB2 gene are implicated as causes of xeroderma pigmentosum group E, an autosomal recessive disease in which patients are defective in nucleotide excision DNA repair. XPE is characterized by hypersensitivity of the skin to sunlight with a high frequency of skin cancer as well as neurologic abnormalities. The hepatitis B virus (HBV) X protein interacts with DDB1, which may mediate HBx transactivation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: DDB1 polyclonal antibody detects endogenous levels of DDB1 protein.
Bioworld Molecular Weight: 127 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution.
Positive control:
Lane 1: HepG2
Lane 2: NIH-3T3
Lane 3: MCF-7
Lane 4: Rat kidney tissue, ICC staining DDB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: DDBa, XAP-1, UV-DDB 1, XPE-BF, XPCe, DDB1, XAP1, Damage specific DNA binding protein 1, Damage-specific DNA-binding protein 1, DDB 1, DDB p127 subunit, Ddb1, DDB1, DDBa, DNA damage binding protein 1, DNA damage-binding protein 1, DNA damage-binding protein a, HBV X-associated protein 1, UV damaged DNA binding factor, UV damaged DNA binding protein 1, UV DDB 1, UV DDB1, UV-damaged DNA-binding factor, UV-damaged DNA-binding protein 1, UV-DDB 1, X associated protein 1, XAP 1, XAP-1, XAP1, Xeroderma pigmentosum group E complementing protein, Xeroderma pigmentosum group E-complementing protein, XPCe, XPE, XPE BF, XPE binding factor, XPE-BF, XPE-binding factor,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified