DDDDK-tag (1A8) Monoclonal Antibody-AP0007M

DDDDK-tag (1A8) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: AP0007M-50, AP0007M-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Host: Mouse

Reactivity: Transfected

Applications: WB, IP, IF

All Applications: WB: 1:5000~1:50000
IP: 1:100~1:1000
IF: 1:100~1:1000

Background: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DDDDK-tag (1A8) mAb detects over-expressed or recombinant proteins containing the DDDDK epitope tag.

Bioworld Molecular Weight: N/A

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DDDDK-tag (1A8) mAb at 1:10000/1:20000/1:30000 dillution
Lane 1: HEK293T whole cell lysate ,untransfected
Lane 2: HEK293T whole cell lysate, transfected with pcDNA3.1-HA-DDDDK-p62, Western blot (WB) analysis of DDDDK-tag (1A8) mAb at 1:5000 dilution
Lane 1: HEK293T whole cell lysate ,untransfected
Lane 2: HEK293T whole cell lysate, transfected with pcDNA3.1-HA-DDDDK-p62 (Anti-SQSTM1, IP)
Lane 3: HEK293T whole cell lysate, transfected with pcDNA3.1-HA-DDDDK-p62 (Anti-DDDDK, IP)
Lane 4: HEK293T whole cell lysate, transfected with pcDNA3.1-HA-DDDDK-p62 (Anti-DDDDK, IP, supernatant), IF image of AP0007M stained HEK293T cells,transfected with pcDNA3.1-p62 #PPL00549-2b. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody DDDDK-tag (1A8) #AP0007M(1:200) at 5µg/ml overnight at +4°C. The secondary antibody (Green) was Goat Anti-Mouse IgG (H+L) FITC#BS50950 used at a 1/1000 dilution for 1h. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).

Alternative Name: DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag,ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; DDDDK

Immunogen: Synthetic peptide DDDDK-tag.

Conjugate: Unconjugated

Modification: Unmodified