DDDDK-tag (1A8) Monoclonal Antibody-HRP-AP0007MH

DDDDK-tag (1A8) Monoclonal Antibody-HRP

Sizes: 50µl, 100µl

Catalogue Numbers: AP0007MH-50, AP0007MH-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB,

All Applications: WB:1:1000~1:10000

Background: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

Purification and Purity: Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase. The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DDDDK-tag (1A8) mAb-HRP detects over-expressed or recombinant proteins containing the DDDDK epitope tag.

Bioworld Molecular Weight: N/A

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DDDDK-tag Mouse Antibody at 1:2000 dilution
Lane 1: HEK293T whole cell lysate, transfected with HA-DDDDK-p62 48h (3uL)
Lane 2: HEK293T whole cell lysate (40ug)

Alternative Name: DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag,ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; DDDDK

Immunogen: Synthetic peptide DDDDK-tag.

Conjugate: HRP

Modification: Unmodified