DDIT3 Polyclonal Antibody-BS90400

DDIT3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90400-50, BS90400-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P35638(Human) P35639(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, FC

All Applications: WB:1:500-1:2,000
FC:1:50-1:100

Background: DDIT3 also named GADD 153 which has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein has also been designated C/EBP-homologous protein (CHOP-10). GADD 153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and metabolic perturbations. GADD 153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD 153 dimers away from "classical" C/EBP binding sites, recognizing instead unique "nonclassical" sites. Thus GADD 153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells, similar to the regulatory function of Id on the activity of Myo D and Myo D-related proteins involved in the development of muscle cells.

Purification and Purity: Peptide affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: DDIT3 polyclonal antibody detects endogenous levels of DDIT3 protein.

Bioworld Molecular Weight: 27 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of DDIT3 on different lysates using anti-DDIT3 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse testis tissue
Lane 2: LOVO
Lane 3: Mouse brain tissue, Flow cytometric analysis of HepG2 cells with DDIT3 antibody at 1/50 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Alternative Name: DDIT-3, CHOP, CHOP-10, DDIT3, CHOP, CHOP10, GADD153, C/EBP homologous protein, C/EBP Homology Protein, C/EBP zeta, C/EBP-homologous protein 10, C/EBP-homologous protein, CCAAT/enhancer binding protein homologous protein, CEBPZ, CHOP 10, CHOP, CHOP-10, CHOP10, DDIT 3, DDIT-3, Ddit3, DDIT3, DNA Damage Inducible Transcript 3, DNA damage-inducible transcript 3 protein, GADD 153, GADD153, Growth Arrest and DNA Damage Inducible Protein 153, Growth arrest and DNA damage inducible protein GADD153, Growth arrest and DNA damage-inducible protein GADD153, MGC4154,

Immunogen: Synthetic peptide within human DDIT3 aa 70-110.

Conjugate: Unconjugated

Modification: Unmodified