DDIT3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90401-50, BS90401-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P35638(Human) P35639(Mouse) Q62857(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC FC
All Applications: WB:1:1,000
FC:1:50-1:100
IHC:1:50-1:200
Background: GADD 153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein has also been designated C/EBP-homologous protein (CHOP-10). GADD 153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and metabolic perturbations. GADD 153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD 153 dimers away from "classical" C/EBP binding sites, recognizing instead unique "nonclassical" sites. Thus GADD 153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells, similar to the regulatory function of Id on the activity of Myo D and Myo D-related proteins involved in the development of muscle cells.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: DDIT3 polyclonal antibody detects endogenous levels of DDIT3 protein.
Bioworld Molecular Weight: 25 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of DDIT3 on PC-12 cells lysates using anti-DDIT3 antibody at 1/1,000 dilution., Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
Alternative Name: CHOP, CHOP-10, DDIT-3, C/EBP homologous protein, C/EBP Homology Protein, C/EBP zeta, C/EBP-homologous protein 10, C/EBP-homologous protein, CCAAT/enhancer binding protein homologous protein, CEBPZ, CHOP 10, CHOP, CHOP-10, CHOP10, DDIT 3, DDIT-3, Ddit3, DDIT3, DNA Damage Inducible Transcript 3, DNA damage-inducible transcript 3 protein, GADD 153, GADD153, Growth Arrest and DNA Damage Inducible Protein 153, Growth arrest and DNA damage inducible protein GADD153, Growth arrest and DNA damage-inducible protein GADD153, MGC4154,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified