Product Description

DDX20 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS61793-50, BS61793-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9UHI6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of motor neurons in the spinal cord. SMA is caused by deletion or loss-of-function mutations in the SMN (survival of motor neuron) gene. Gemin3, also known as DP103, DDX20, DEAD-box protein DP130 and DEAD/H box 20, is a protein product of human chromosome 1p13.2. It associates directly with SMN and is a part of the SMN complex containing Gemin2, Gemin4, Gemin5 and Gemin6, as well as several spliceosomal snRNP proteins. The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing of the nucleus. It is found in both the cytoplasm and the nucleus. The nuclear form is concentrated in subnuclear bodies called gems (for Gemini of the coiled bodies). Gemin3 also interacts with SmB, SmD2 and SmD3. It contains the conserved motif Asp-Glu-Ala-Asp (DEAD) characteristic of DEAD-box proteins. Gemin3 is a putative RNA helicase and shows ATPase activity. It is expressed in B and T cell neuroblastoma-derived cell lines, malignant melanoma tumor, normal testis and is expressed in low levels in colon, skeletal muscle, liver, kidney and lung.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: DDX20 pAb detects endogenous levels of DDX20 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DDX20 pAb at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: HCC827 whole cell lysate (40ug)
Lane 3: H9C2 whole cell lysate (40ug)
Lane 4: CT26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of DDX20 polyclonal antibody in paraffin-embedded human colorectal cancer tissue at 1:50.

Bioworld Molecular Weight: ~ 100 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Probable ATP-dependent RNA helicase DDX20; Component of gems 3; DEAD box protein 20; DEAD box protein DP 103; Gemin-3; DDX20; GEMIN3

Immunogen: Synthetic peptide, corresponding to amino acids 270-320 of Human DDX20.

Conjugate: Unconjugated

Modification: Unmodified