Product Description

DDX21 Polyclonal Antibody

Catalogue Numbers:BS75971-50, BS75971-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NR30

Host: Rabbit

Reactivity: Human, Mouse

Applications: IHC, IF/ICC

All Applications: IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry of paraffin-embedded mouse testis using DDX21 antibody at dilution of 1:100.Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse brain using DDX21 antibody at dilution of 1:100.Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunofluorescence analysis of U-2 OS cells using DDX21 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Confocal immunofluorescence analysis of U2OS cells using DDX21 Polyclonal antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: DDX21;GUA;GURDB;RH-II/GU;RH-II/GuA

Immunogen: Recombinant fusion protein of human DDX21(NP_004719.2).

Conjugate: Unconjugated

Modification: Unmodified