DDX3 (6G8) Monoclonal Antibody-MB0178

DDX3 (6G8) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0178-50, MB0178-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O00571

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IP,

All Applications: WB: 1:1000
IP: 1:50~200
ICC: 1:50~200

Background: DDX3 contains all of the motifs of the DEAD box family of RNA helicases, including the Asp/Glu/Ala/Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It is also involved in cell growth control and is deregulated in hepatitis virus associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of DDX3 and does not cross-react with related proteins.

Bioworld Molecular Weight: Predicted band size: 73KDa
Observed band size: 73KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot detection of DDX3 in Hela,3T3,C6,COS7,K562 and Jurkat cell lysate using DDX3 mouse mAb (1:1000 diluted)., Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using DDX3 mouse mAb (dilution 1:200)., Immunoprecipitation analysis of Hela cell lysates using DDX3 mouse mAb.

Alternative Name: ATP-dependent RNA helicase DDX3X; DEAD box protein 3, X-chromosomal; DEAD box, X isoform; Helicase-like protein 2; HLP2; DDX3X; DBX; DDX3

Immunogen: Purified recombinant human DDX3 protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified