Product Description

DDX3 (S63) Polyclonal Antibody

Catalogue Numbers: BS3224-50, BS3224-100

Sizes: 50µl, 100µl

Alternative Name: ATP-dependent RNA helicase DDX3X; DEAD box protein 3, X-chromosomal; DEAD box, X isoform; Helicase-like protein 2; HLP2; DDX3X; DBX; DDX3

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O00571

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: DDX3 contains all of the motifs of the DEAD box family of RNA helicases, including the Asp/Glu/Ala/Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It is also involved in cell growth control and is deregulated in hepatitis virus associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DDX3 (S63) polyclonal antibody detects endogenous levels of DDX3 protein.

Bioworld Molecular Weight: ~ 75 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DDX3 (S63) pAb at 1:500 dilution
Lane 1: PC3 whole cell lysate(40ug)
Lane 2: A549 whole cell lysate(40ug)
Lane 3: HepG2 whole cell lysate(40ug)
Lane 4: C6 whole cell lysate(40ug)
Lane 5: AML-12 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 41-90 of Human DDX3.

Conjugate: Unconjugated

Modification: Unmodified