Product Description

DDX3X Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66592-50, MB66592-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The DEAD box family of RNA helicases is characterized in part by a common D-E-A-D amino acid motif. The family is composed of a growing number of proteins found in a wide range of organisms from bacteria to mammals. DEAD helicases have distinct biological functions in RNA metabolism and ribonucleoprotein (RNP) processing (reviewed in 1, 2). DDX3 is a DEAD box family RNA helicase with diverse cellμlar functions. DDX3 is required for nuclear export of HIV-1 viral transcripts, possibly in a complex with the viral Rev protein and host cofactor CRM1. DDX3 is required for hepatitis C virus (HCV) RNA replication and its expression is downregμlated in hepatitis B virus (HBV) associated hepatocellμlar carcinoma (HCC). Recent evidence suggests that DDX3 functions as a tumor suppressor protein. Its expression inhibits tumor cell colony formation and increases expression of the cdk inhibitor p21 Waf1/Cip1. Low DDX3 expression has been shown in HCC, and aberrant subcellμlar localization occurs in many squamous cell carcinomas. Reduced DDX3 expression in cμltured cells causes a diminished dependence on serum for cell proliferation and changes in cyclin D1 and p21 Waf1/Cip1 expression. DDX3 is phosphorylated at Thr204 and Thr323 by the mitotic cyclin dependent kinase, cyclin B/cdc2. This phosphorylation is thought to cause a loss of DDX3 function and a concomitant repression of ribosome biogenesis and translation in mitosis.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Applications: WB, IF/ICC, IP, ChIP

Alternate Names: DBX, DDX3, ATP-dependent RNA helicase DDX3X, DEAD box protein 3 X-chromosomal, DEAD box X isoform, Helicase-like protein 2, HLP2

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human DDX3 protein fragments expressed in E.coli.

Dilution: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50), ChIP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of DDX3X protein.

Molecular Weight: ~ 75 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O00571

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only