DDX5 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90403-50, BS90403-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P17844(Human) Q61656(Mouse)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: p68 RNA helicase is a nuclear protein that exhibits RNA-dependent ATPase activity. Phosphorylation by protein kinase C inhibits p68 RNA helicase activity. p68 RNA helicase appears to play a role in organ differentiation during development. Furthermore, p68 RNA helicase is expressed in early neural development and in various mesodermal tissues in a number of different chordate embryos. At the cellular level, the expression levels of p68 RNA helicase increases in serum-induced quiescent cell lines. p68 RNA helicase may function as a coactivator for estrogen receptor alpha. Additionally, p68 RNA helicase associates with transcriptional coactivators CBP and p300. p68 RNA helicase localizes to the nucleus under normal conditions. During late telophase, p68 RNA helicase and fibrillarin colocalize to nascent nucleoli. p68 RNA helicase may function as a heterodimer with p72 RNA helicase.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: DDX5 polyclonal antibody detects endogenous levels of DDX5 protein.
Bioworld Molecular Weight: 69/60 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of DDX5 on different cell lysate using anti-DDX5 antibody at 1/1,000 dilution.
Positive control:
Lane 1: PC-12
Lane 2: MEF
Lane 3: SH-SY5Y
Lane 4: NIH-3T3
Lane 5: Mouse brain, ICC staining DDX5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: G17P1, HELR, HLR1, ATP dependent RNA helicase DDX5, DDX 5, Ddx5, DDX5, DEAD (Asp Glu Ala Asp) box helicase 5, DEAD (Asp Glu Ala Asp) box polypeptide 5, DEAD box 5, DEAD box protein 5, DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD), G17P1, HELR, HLR1, HUMP68, P68, p68 RNA helicase, Probable ATP dependent RNA helicase DDX5, Probable ATP-dependent RNA helicase DDX5, RNA helicase p68,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified