DDX6 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90404-50, BS90404-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P26196(Human) P54823(Mouse)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB:1:500-1:1,000
IHC:1:50-1:100
Background: RCK, also known as DDX6 and P54, is a member of the DEAD-box RNA helicase family of proteins, all of which share common protein motifs. Found in most tissues, RCK is an unwindase that exhibits ATP-dependent RNA un-winding activity, as well as the ability to decay RNA in the 5'-3' direction. In non-malignant cells, RCK is associated with all processes of normal RNA metabolism including splicing, export and translation initiation. Mutations in the gene encoding RCK can cause the protein to be overexpressed, changing its function to that of an oncogene that positively regulates the expression of genes involved in cell growth and proliferation. It is believed that, through its unwindase activity, the main function of RCK is to downregulate mRNA expression and maintain normal transcriptional levels within the cell.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: DDX6 polyclonal antibody detects endogenous levels of DDX6 protein.
Bioworld Molecular Weight: 54 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of DDX6 on different lysates using anti-DDX6 antibody at 1/500 dilution.
Positive control:
Lane 1: Human colon
Lane 2: SH-SY-5Y
Lane 3: SiHa
Lane 4: K562, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-DDX6 antibody. Counter stained with hematoxylin.
Alternative Name: DDX6, HLR2, RCK, ATP dependent RNA helicase DDX6, ATP dependent RNA helicase p54, DDX6, DEAD (Asp Glu Ala Asp) box polypeptide 6, DEAD box 6, DEAD box protein 6, DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RNA helicase 54kD), FLJ36338, HLR2, Oncogene RCK, P54, Probable ATP-dependent RNA helicase DDX6, RCK,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified