Product Description

Desmin Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66067-50, MB66067-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments and microtubμles. Major types of intermediate filaments are distinguished and expressed in particμlar cell types: cytokeratins (epithelial cells), glial fibrillary acidic protein or GFAP (glial cells), desmin (skeletal, visceral and certain vascμlar smooth muscle cells), vimentin (mesenchyme origin) and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modμlate their motility and shape. In particμlar, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes. Vimentin is present in sarcomas, but not carcinomas, and its expression is examined relative to other markers to distinguish between the two forms of neoplasm. Desmin is a myogenic marker expressed in early development that forms a network of filaments that extends across the myofibril and surrounds Z discs. The desmin cytoskeleton provides a connection among myofibrils, organelles and the cytoskeleton. Desmin knockout mice develop cardiomyopathy, skeletal and smooth muscle defects. In humans, desmin related myopathies might be caused by mutations in the corresponding desmin gene or in proteins with which desmin interacts, including αB-crystallin and synemin. Disorganized desmin filaments and the accumμlation of protein aggregates comprised predominantly of desmin characterize desmin-related myopathies.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: IHC

Alternate Names: Desmin

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Desmin. The exact sequence is proprietary.

Dilution: IHC (1/100 - 1/300)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of Desmin protein.

Molecular Weight: ~ 53 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P17661

Product: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Research Use Only