Product Description

DFF45 (ICAD) Mouse Monoclonal Antibody

Sizes: 25μl, 100μl

Catalogue Numbers: MB4004-25, MB4004-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: IF, WB

Alternate Names: DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit (DFF-45), Inhibitor of CAD (ICAD)

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Fμll length human recombinant protein of human DFFA (NP_004392) produced in E.coli.

Dilution: WB 1:2500~5000, IF 1:50~100

Purification: The antibody was affinity-purified from mouse ascites fluids or tissue cμlture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IgG1

Molecular Weight: ~ 45 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O00273

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only