DFFA Polyclonal Antibody
Catalogue Numbers: BS60819-50, BS60819-100
Sizes: 50µl, 100µl
Alternative Name: DNA fragmentation factor subunit alpha; DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD; DFFA; DFF1; DFF45; H13
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O00273
Host: Rabbit
Reactivity: Human, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:203
Background: The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADP-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins. PARP is a nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature apoptotic fragment. Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca++-independent manner. In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites. DFF-45/ICAD, the subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation. The nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -23°C long term. Avoid freeze-thaw cycles.
Specificity: DFFA polyclonal antibody detects endogenous levels of DFFA protein.
Bioworld Molecular Weight: ~ 12 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of DFFA polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: H9C2 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human DFFA.
Conjugate: Unconjugated
Modification: Unmodified