DFFB Polyclonal Antibody
Catalogue Numbers: BS60830-50, BS60830-100
Sizes: 50µl, 100µl
Alternative Name: DNA fragmentation factor subunit beta (EC:3.-.-.-); Caspase-activated deoxyribonuclease; CAD; Caspase-activated DNase; CPAN; DNA fragmentation factor 40 kDa subunit; DFF-40; DFFB
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O76075
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: The Ced/ICE or caspase family of cysteine proteases plays a pivotol role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly(ADP-ribose) polymerase (PARP), Gelsolin, ICAD (inhibitor of CAD, also designated DFF-45) and the nuclear lamins. CAD (caspase-activated deoxyribonuclease), also designated CPAN (caspase-activated nuclease) and DFF40, is a DNase that is responsible for DNA degradation during apoptosis. Caspase-3 acts to dissociate CAD from ICAD, allowing CAD to enter the nucleus and degrade DNA.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: DFFB polyclonal antibody detects endogenous levels of DFFB protein.
Bioworld Molecular Weight: ~ 39 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of DFFB polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: H9C2 whole cell lysate
Lane 4: Raw264.7 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human DFFB.
Conjugate: Unconjugated
Modification: Unmodified