DFFB Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A10110-20, A10110-100
Citations, Manuals and MSDS Available upon request.
Background: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, ELISA
Cross-reactivity: Human, Mouse
Protein Weight: 39kDa
Observed Molecular Weight: 39kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 1677
SWISS: O76075
Alternate Names: CAD; CPAN; DFF2; DFF40; DFF-40; DFFB
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:200 - 1:1000 IHC-P, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics Nuclear Signaling, Cell Biology Developmental Biology, Apoptosis, Cell Cycle, Death Receptor Signaling Pathway, Endocrine Metabolism.
NCBI Alias: DFFB
Research Use Only