DGK-α (A340) Polyclonal Antibody-BS1960

DGK-α (A340) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1960-50, BS1960-100

Sizes: 50µl, 100µl

Swiss-Prot: P23743

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC

Background: Diacylglycerol kinases (DGKs) phosphorylate diacylglycerol (DAG) to produce phosphatidic acid. DAG and phosphatidic acid are lipids that act as second messengers in signaling cascades. DGK-alpha influences cell activation and secretion of lethal exosomes, which in turn control cell death. DGK-beta is abundant in restricted brain regions such as the caudate putamen and olfactory tubercle. DGK-gamma encodes full-length and truncated transcripts that are present in a range of human tissues, with greatest expression observed in retina. DGK-delta is most abundant in skeletal muscle. DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol and is expressed predominantly in testis. DGK-zeta is most abundant in brain and muscle. DGK-eta is closely related to DGK-delta. DGK-theta is most abundant in the cerebellum and hippocampus.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: DGK-α (A340) polyclonal antibody detects endogenous levels of DGK-α protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DGK-α (A340) pAb at 1:2000 dilution
Lane 1: C6 whole cell lysate (40ug)
Lane 2: U-87MG whole cell lysate (40ug)
Lane 3: Myla2059 whole cell lysate (40ug)
Lane 4: HuT78 whole cell lysate (10ug), Immunohistochemistry (IHC) analyzes of DGK-α (A340) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 90 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Diacylglycerol kinase alpha; DAG kinase alpha; 80 kDa diacylglycerol kinase; Diglyceride kinase alpha; DGK-alpha; DGKA; DAGK; DAGK1

Immunogen: Synthetic peptide, corresponding to amino acids 310-360 of Human DGK-α.

Conjugate: Unconjugated

Modification: Unmodified