Product Description

DHS (L87) Polyclonal Antibody

Catalogue Numbers: BS3847-50, BS3847-100

Sizes: 50µl, 100µl

Alternative Name: Deoxyhypusine synthase; DHPS; DS

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49366

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Deoxyhypusine synthase (DHS) is crucial for the posttranslational formation of hypusine, a modification of a specific lysine residue in eukaryotic initiation factor 5A (eIF-5A). Hypusine is formed by posttranslational modifications involving two enzymatic steps catalyzed by DHS and deoxyhypusine hydroxylase (DOHH). eIF-5A is essential for eukaryotic cell proliferation. DHS, which belongs to the deoxyhypusine synthase family of proteins, is important for the first step in the hypusine biosynthesis pathway. It acts as a catalyst for the NAD-dependent oxidative cleavage of spermidine and the ensuing transfer of the butylamine moiety of spermidine to the eIF-5A protein, to create the intermediate deoxyhypusine residue.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DHS (L87) polyclonal antibody detects endogenous levels of DHS protein.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DHS (L87) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate(40ug)
Lane 2: CT26 whole cell lysate(40ug)
Lane 3: PC12 whole cell lysate(40ug)
Lane 4: PC3 whole cell lysate(40ug)
Lane 5: MCF-7 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human DHS.

Conjugate: Unconjugated

Modification: Unmodified