DIAPH2 Polyclonal Antibody
Catalogue Numbers: BS60413-50, BS60413-100
Sizes: 50µl, 100µl
Alternative Name: Protein diaphanous homolog 2; Diaphanous-related formin-2; DRF2; DIA; Diap2; Dia2; Dia 2
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O60879
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Dia 1 (also designated p140mDia) and Dia 2 (also designated p134mDia 2), mammalian homologs of the Drosophila diaphanous gene, belong to a family of formin homology (FH) proteins which are characterized by having tandemly aligned FH1 and FH2 domains in their carboxy terminal regions. The amino terminus of inactivated Dia 1 binds to the FH1 and FH2 domains and is activated when GTP-bound Rho disrupts the intramolecular interaction of Dia 1 and converts it to an open conformation. Once activated, Dia 1 co-localizes with Profilin and RhoA in dynamic plasma membrane structures and induces the formation of stress fibers in conjunction with the ROCK-mediated signaling pathway. However, Dia 1 and ROCK activities antagonize each other in order to determine the thickness and density of the stress fibers formed.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: DIAPH2 polyclonal antibody detects endogenous levels of DIAPH2 protein.
Bioworld Molecular Weight: ~ 130 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of DIAPH2 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human DIAPH2.
Conjugate: Unconjugated
Modification: Unmodified