Product Description

DiMethyl-DNMT3A-K44 Polyclonal Antibody

Catalogue Numbers:BS74110-50, BS74110-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O88508

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IF/ICC

All Applications: IF/ICC,1:50 - 1:200

Background: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Methylated

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunofluorescence analysis of U2OS cells using DiMethyl-DNMT3A-K44 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of C6 cells using DiMethyl-DNMT3A-K44 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of HeLa cells using DiMethyl-DNMT3A-K44 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using DiMethyl-DNMT3A-K44 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: Dnmt3a;MmuIIIA;DNMT3A2;M.HsaIIIA;TBRS

Immunogen: A synthetic dimethylated peptide around K44 of mouse DNMT3A(NP_031898.1).

Conjugate: Unconjugated

Modification: Methylated