Dio-1 (S186) Polyclonal Antibody-BS2533

Dio-1 (S186) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2533-50, BS2533-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9BTC0

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Dio-1 (Death Inducer-Obliterator-1) is a putative transcription factor that contains two zinc finger motifs. Dio-1 translocates to the nucleus, and activates apoptosis during limb development. Programmed cell death, a highly regulated form of apoptosis, plays an important role in determining the amount of tissue, the shape, and the definition of each digit during limb development. Dio-1 expression is upregulated when an apoptotic signal is detected, and subsequently apoptosis is induced. This process is similar to the expression of NFκB and NGF in response to external signals. Dio-1 expression is suppressed by caspase inhibitors and Bcl-2 expression. This supports the theory that Dio-1 functions in the onset of programmed cell death.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Dio-1 (S186) polyclonal antibody detects endogenous levels of Dio-1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Dio-1 (S186) pAb at 1:500 dilution
Lane 1: PC3 whole cell lysate (20ug)
Lane 2: HEK293T whole cell lysate (20ug)
Lane 3: C6 whole cell lysate (40ug)
Lane 4: CT26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Dio-1 (S186) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 244 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Death-inducer obliterator 1; DIO-1; hDido1; Death-associated transcription factor 1; DATF-1; DIDO1; C20orf158; DATF1; KIAA0333

Immunogen: Synthetic peptide, corresponding to amino acids 154-198 of Human Dio-1.

Conjugate: Unconjugated

Modification: Unmodified