DIO3 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A6900-20, A6900-100
Citations, Manuals and MSDS Available upon request.
Background: The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3, 3', 5-triiodothyronine (T3) to inactive metabolites, 3, 3', 5'-triiodothyronine (RT3) and 3, 3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in pregnant uterus, placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It regulates circulating fetal thyroid hormone concentrations, and thus plays a critical role in mammalian development. Knockout mice lacking this gene exhibit abnormalities related to development and reproduction, and increased activity of this enzyme in infants with hemangiomas causes severe hypothyroidism. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
Category: Polyclonal Antibodies
Applications: WB, ELISA
Cross-reactivity: Human
Protein Weight: 34kDa
Observed Molecular Weight: 34kDa
Immunogen: Recombinant protein (or fragment) .
Species: Human
GeneID: 1735
SWISS: P55073
Alternate Names: D3; 5DIII; TXDI3; DIOIII; DIO3
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:2000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Cancer, Signal Transduction, Cell Biology Developmental Biology, Growth factors, Endocrine Metabolism, Neuroscience.
NCBI Alias: DIO3
Research Use Only