Dipeptidyl Peptidase IV (DPP4) Activity Fluorometric Assay Kit - E-BC-F053

Dipeptidyl Peptidase IV (DPP4) Activity Fluorometric Assay Kit

Size: 96T

Catalogue Number: E-BC-F053-96

Citations, Manuals and MSDS Available upon request.

Target Synonym: DPP4

Research Areas: Amino Acids and Proteins

Result Type: Enzyme Activity

Detection Instrument: Fluorescence microplate reader

Tested Sample Types: serum, plasma, tissue and cell

Assay Time: 70 min

Detection Type: Fluorescence method

Sensitivity: 0.92 U/L

Detection Range: 0.92-3.77 U/L

Test Principle: Dipeptidyl Peptidase IV (DPP4) , also known as CD26, is a kind of serine protease, can decompose the second N-terminal proline or alanine residue of the peptide chain. In organisms, dipeptidyl peptidase can rapidly decompose incretin, which can stabilize insulin level and promote the reduction of blood glucose level in organisms. The detection principle of this kit is that DPP4 can decompose the substrate and release the fluorescent substance AMC, and the activity of this enzyme can be inhibited by adding DPP4 inhibitor. The activity of DPP4 could be calculated by the difference of fluorescence value before and after inhibition.

Inter CV: 5.7

Intra CV: 0.6

Storage: This product can be stored at -20°C for 12 months with shading light.

Shipping: Ice bag