DIS3L Polyclonal Antibody-BS72855

DIS3L Polyclonal Antibody Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Sizes: 50 µl, 100 µl Catalogue Numbers: BS72855-50, BS72855-100 Swiss-Prot: Q8TF46 Host: Rabbit DIS3L Polyclonal Antibody-BS72855 Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Reactivity: Human, Mouse, Rat Applications: WB, IHC All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 Background: The cytoplasmic RNA exosome complex degrades unstable mRNAs and is involved in the regular turnover of other mRNAs. The protein encoded by this gene contains 3' -5' exoribonuclease activity and is a catalytic component of this complex. Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Specificity: Polyclonal Antibodies Bioworld Molecular Weight: 130kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of extracts of various cell lines, using DIS3L antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s., Immunohistochemistry of paraffin-embedded Human liver cancer using DIS3L Rabbit pAb at dilution of 1:100.Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol. Alternative Name: DIS3L; DIS3L1 Immunogen: Recombinant fusion protein of human DIS3L(NP_588616.1). Modification: Unmodified Conjugate: Unconjugated