Product Description

DLC1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67463-50, BS67463-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q96QB1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Background: Loss of expression of deleted in liver cancer 1 (DLC-1) protein correlates strongly with cancerous phenotype in a large number of human tissues, such as breast, liver, colon and prostate, and generally occurs due to genomic deletion or aberrant promotor methylation. The gene encoding DLC-1 maps to human chromasome 8p22, a region presumed to harbor tumor supressor genes based on its frequent mutation in a large number of cancers. DLC-1 localizes to the cytoplasm and restored expression leads to caspase-3 mediated apoptosis, and inhibition of cell growth and invasiveness.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of DLC1 protein.

Bioworld Molecular Weight: ~ 175 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of DLC1 expression in C6 (A), CT26 (B), LOVO (C), HEK293T (D) whole cell lysates., Immunohistochemical analysis of DLC1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of DLC1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: ARHGAP7; KIAA1723; STARD12; Rho GTPase-activating protein 7; Deleted in liver cancer 1 protein; DLC-1; HP protein; Rho-type GTPase-activating protein 7; START domain-containing protein 12; StARD12; StAR-related lipid transfer protein 12

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DLC1. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified