Product Description

DMPK (R30) Polyclonal Antibody

Catalogue Numbers: BS2298-50, BS2298-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q09013

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Myotonic dystrophy protein kinase (DMPK) is a ulti-domain protein kinase found in muscle that is activated in response to G protein second messengers and proteolysis. DMPK is implicated in myotonic muscular dystrophy (DM), an autosomal dominant-inherited disorder that predominately affects skeletal and cardiac muscle and causes defects in cardiac conduction. DM arises through expansion of CTG repeats in the 3’-UTR of the DMPK gene. Mutant DMPK transcripts with an extended region of CUG repeats are retained in the nucleus. These transcripts also influence the expression of the DM locus-associated homeodomain protein (DMAHP)/SIX5, to mediate in part the DM phenotype. Other substrates for DMPK include myogenin, Ltype calcium channels, and Phospholemman (PLM).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DMPK (R30) polyclonal antibody detects endogenous levels of DMPK protein.

Extra Notes: Western blot (WB) analysis of DMPK (R30) pAb at 1:500 dilution
Lane1:CT26 whole cell lysate(40ug)
Lane2:The Brain tissue lysate of Rat(40ug)
Lane3:A549 whole cell lysate(40ug)
Lane4:L02 whole cell lysate(40ug)
Lane5:HEK293T whole cell lysate(20ug)

Bioworld Molecular Weight: ~ 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Myotonin-protein kinase; MT-PK; DM-kinase; DMK; DM1 protein kinase; DMPK; Myotonic dystrophy protein kinase; DMPK; DM1PK; MDPK

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human DMPK.

Conjugate: Unconjugated

Modification: Unmodified