Product Description

DNA Ligase I Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90416-50, BS90416-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P18858(Human) 37227(Rat)

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC

All Applications: WB:1:500
IHC:1:50-1:200

Background: This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: DNA Ligase I polyclonal antibody detects endogenous levels of DNA Ligase I protein.

Bioworld Molecular Weight: Predicted band size 102 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of DNA Ligase I on different cell lysates using anti-DNA Ligase I antibody at 1/500 dilution.
Positive control:
Lane 1: Daudi
Lane 2: A431
Lane 3: MCF-7, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-DNA Ligase I antibody. Counter stained with hematoxylin.

Alternative Name: LIG1, DNA ligase 1, DNA ligase I, DNLI1, LIG 1, lig1, Ligase I DNA ATP dependent, MGC117397, MGC130025, Polydeoxyribonucleotide synthase [ATP] 1,

Conjugate: Unconjugated

Modification: Unmodified