Product Description

DNA Ligase IV (G619) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS2234-50, BS2234-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The X-ray repair cross-complementing protein XRCC4 and DNA Ligase IV are essential for repairing double-strand breaks in DNA. These proteins form a critical complex consisting of two molecμles of each protein that preferentially bind DNA with nicks or broken ends. As an obligate accessory molecμle, XRCC4 binds to DNA Ligase IV and enhances its joining activity. The XRCC4/ DNA Ligase IV complex is also involved in V(D)J recombination. V(D)J recombination occurs in normal development of the adaptive immune system and involves the formation of a double-strand break intermediate. Deletions of either DNA Ligase IV or XRCC4 inhibit the completion of V(D)J recombination, resμlting in a high incidence of apoptosis in the developing nervous system and a block in B and T cell maturation.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Rabbit

Applications: IHC, IF

Alternate Names: DNA ligase IV ATP dependent, DNA joinase, DNA ligase 4, DNA ligase IV, DNA repair enzyme, DNLI4, LIG 4, LIG4, Ligase IV, Ligase IV DNA ATP dependent, Polydeoxyribonucleotide synthase [ATP] 4, Polydeoxyribonucleotide synthase 4, Polydeoxyribonucleotide synthase, Polynucleotide ligase, Sealase,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 600-650 of Human DNA Ligase IV.

Dilution: IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: DNA Ligase IV (G619) polyclonal antibody detects endogenous levels of DNA Ligase IV protein.

Molecular Weight: ~ 104 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P49917

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only