DNA Ligase IV Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90417-50, BS90417-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P49917(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, IHC ICC
All Applications: WB:1:500-1:2,000
IHC:1:50-1:200
ICC:1:10-1:50
Background: The X-ray repair cross-complementing protein XRCC4 and DNA Ligase IV are essential for repairing double-strand breaks in DNA. These stories form a critical complex composed of two molecules of each protein that preferentially bind DNA with nicks or broken ends. As an obligatory accessory molecule, XRCC4 binds to DNA Ligase IV and enhances its joining activity. The XRCC4 / DNA Ligase IV complex is also involved in V (D) J recombination. V (D) (in) a pair of high incidence of apoptosis in the development nervous system and a block in B and T cell maturation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: DNA Ligase IV polyclonal antibody detects endogenous levels of DNA Ligase IV protein.
Bioworld Molecular Weight: 104 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of DNA Ligase IV on Hela cell using anti-DNA Ligase IV antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Hela
Lane 3: Jurkat, Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-DNA Ligase IV antibody. Counter stained with hematoxylin.
Alternative Name: LIG4, DNA ligase IV ATP dependent, DNA joinase, DNA ligase 4, DNA ligase IV, DNA repair enzyme, DNLI4, LIG 4, LIG4, LIG4S, Ligase IV, Ligase IV DNA ATP dependent, Polydeoxyribonucleotide synthase [ATP] 4, Polydeoxyribonucleotide synthase 4, Polydeoxyribonucleotide synthase, Polynucleotide ligase, Sealase,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified