Product Description

DNA Ligase IV Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA24245-50, RA24245-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: DNA ligase 4 (LIG4) Homo sapiens The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V (D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008],

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB, IHC-p, IF/ICC, ELISA

Alternate Names: LIG4, DNA ligase 4, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4

Clonality: Polyclonal

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight: 103

Protein Molecular Weight (kDa): 103

Storage: -20°C/1 year

Human Gene ID: 3981

Human SwissProt Number: P49917

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Research Use Only