DNA Ligase IV Polyclonal Antibody
Sizes: 50μL, 100μL
Catalogue Numbers: RA24246-50, RA24246-100
Citations, Manuals and MSDS Available upon request.
Background: DNA ligase 4 (LIG4) Homo sapiens The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V (D) J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ) . This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK) . Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008],
Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions: -20°C/1 year
Recommended dilutions: WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Alternative Names: LIG4; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
Applications: IHC-p; IF (paraffin section) ; WB; ELISA
Species Cross-Reactivity: Human; Rat; Mouse
GeneID (Human): 3981
Protein MW (KDa): 133
SWISS: P49917
Source: Rabbit
Research Use Only