Product Description

DNA pol α (K111) Polyclonal Antibody

Catalogue Numbers: BS1922-50, BS1922-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P09884

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases. DNA pol α, β, δ and ε are involved in DNA replication and repair. DNA pol δ and DNA pol ε are multisubunit enzymes, with DNA pol δ consisting of two subunits: p125, which interacts with the sliding DNA clamp protein PCNA; and p50. The nuclear-encoded DNA pol γ is the only DNA polymerase required for the replication of the mitochondrial DNA. DNA pol ζ is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis. DNA pol θ is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DNA pol α (K111) polyclonal antibody detects endogenous levels of DNA pol α protein.

Extra Notes: Western blot (WB) analysis of DNA pol α (K111) pAb at 1:1000 dilution
Lane1:HCT116 whole cell lysate(40ug)
Lane2:EC9706 whole cell lysate(40ug)
Lane3:SGC7901 whole cell lysate(40ug)
Lane4:HEK293T whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 180 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; POLA1; POLA; DNA polα

Immunogen: Synthetic peptide, corresponding to amino acids 81-130 of Human DNA pol α.

Conjugate: Unconjugated

Modification: Unmodified