DNA topoisomerase I (TOP1) Polyclonal Antibody-BS70887

DNA topoisomerase I (TOP1) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS70887-50, BS70887-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11387

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IP,

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IP,1:20 - 1:50

Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 120kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using DNA topoisomerase I) antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunohistochemistry of paraffin-embedded mouse liver using DNA topoisomerase I) antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug DNA topoisomerase I) antibody. Western blot was performed from the immunoprecipitate using DNA topoisomerase I) antibody at a dilution of 1:1000.

Alternative Name: TOP1; TOPI

Immunogen: A synthetic peptide of human DNA topoisomerase I (DNA topoisomerase I (TOP1))(NP_003277.1).

Conjugate: Unconjugated

Modification: Unmodified