Product Description

DNA2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79221-50, BS79221-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51530

Host: Rabbit

Reactivity: Mouse

Applications: WB, IHC

All Applications: WB,1:500 - 1:1000 | IHC,1:20 - 1:50

Background: This gene encodes a member of the DNA2/NAM7 helicase family. The encoded protein is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability. Mutations in this gene are associated with autosomal dominant progressive external ophthalmoplegia-6 (PEOA6) and Seckel syndrome 8. Alternatively spliced transcript variants have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 127kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Mouse liver, using DNA2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s.

Alternative Name: DNA2; DNA2L; hDNA2

Immunogen: Recombinant fusion protein of human DNA2(NP_001073918.2).

Conjugate: Unconjugated

Modification: Unmodified