Product Description

DNMT1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80296-50, BS80296-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P26358

Host: Rabbit

Reactivity: Human

Applications: WB, IF/ICC, IP, , ChIP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:200 | ChIP,1:50 - 1:200

Background: This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using DNMT1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of 293T cells using DNMT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug DNMT1 antibody. Western blot was performed from the immunoprecipitate using DNMT1 at a dilution of 1:1000., Chromatin immunoprecipitation analysis of extracts of Jurkat cells, using DNMT1 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Alternative Name: DNMT1; ADCADN; AIM; CXXC9; DNMT; HSN1E; MCMT; m.HsaI; DNA methyltransferase 1

Immunogen: Recombinant fusion protein of human DNMT1(NP_001370.1).

Conjugate: Unconjugated

Modification: Unmodified