DNMT3A Polyclonal Antibody-BS6587

DNMT3A Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS6587-50, BS6587-100

Swiss-Prot: Q9Y6K1

Host: Rabbit

DNMT3A Polyclonal Antibody-BS6587

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IP,

All Applications: WB,1:500 - 1:2000 | IP,1:50 - 1:200

Background: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 110kDa/130KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using DNMT3A antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST., Immunoprecipitation analysis of 200ug extracts of A-549 cells using 3ug DNMT3A antibody. Western blot was performed from the immunoprecipitate using DNMT3A antibody at a dilution of 1:1000.

Alternative Name: DNMT3A; DNMT3A2; M.HsaIIIA; TBRS; DNMT3A

Immunogen: Recombinant fusion protein of human DNMT3A(NP_072046.2).

Modification: Unmodified

Conjugate: Unconjugated