Product Description

DOCK 180 (K1692) Polyclonal Antibody

Catalogue Numbers: BS3846-50, BS3846-100

Sizes: 50µl, 100µl

Alternative Name: DOCK180; DOCK-180; dedicator of cytokinesis; 180 kDa; DOCK1; DOCK-1; dedicator of cytokinesis 1; ced5

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14185

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: DOCK 180, a 180 kDa protein downstream of Crk, has been identified as a major Crk-associated protein. When DOCK 180 is recruited to the plasma membrane from a cytoplasmic reservoir, presumably by Crk, changes in cellular morphology and spindle formation occur, suggesting DOCK 180 to be a Crk effector molecule

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DOCK 180 (K1692) polyclonal antibody detects endogenous levels of DOCK 180 protein.

Bioworld Molecular Weight: ~ 215 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DOCK1 pAb at 1:500 dilution
Lane 1: MCF-7 whole cell lysate(40ug)
Lane 2: H1792 whole cell lysate(40ug)
Lane 3: C6 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 1660-1719 of Human DOCK 180.

Conjugate: Unconjugated

Modification: Unmodified