DOCK2 Polyclonal Antibody
Catalogue Numbers: BS61172-50, BS61172-100
Sizes: 50µl, 100µl
Alternative Name: Dedicator of cytokinesis protein 2; DOCK2; KIAA0209
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q92608
Host: Rabbit
Reactivity: Human, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: The DOCK2 gene encodes dedicator of cytokinesis 2 (DOCK 2), a hematopoietic cell-specific CDM family protein that is indispensable for lymphocyte chemotaxis. DOCK 2 participates in the cytoskeletal rearrangements that are required for lymphocyte migration in response of chemokines. This peripheral membrane protein activates Rac 1 and Rac 2 small GTPases, while presumably acting as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK 2 may also participate in IL-2 transcriptional activation through the activation of Rac 2. DOCK 2 contains one DHR-1 (CZH-1) domain, one DHR-2 (CZH-2) domain and one SH3 domain. The DHR-2 domain is a putative GEF activity mediator. The DOCK 2 protein also co-localizes with F-Actin, and demonstrates expression in several human tissues, with the highest levels observed in peripheral blood leukocytes, thymus, spleen and liver.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: DOCK2 pAb detects endogenous levels of DOCK2 protein.
Bioworld Molecular Weight: ~ 211 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of DOCK2 pAb at 1:500 dilution
Lane 1: The Thymus tissue lysate of Rat(10ug)
Lane 2: Myla2059 whole cell lysate(40ug)
Lane 3: K562 whole cell lysate(40ug)
Immunogen: Synthetic peptide, corresponding Human DOCK2.
Conjugate: Unconjugated
Modification: Unmodified