Product Description

Dok-1 (phospho-Y362) Polyclonal Antibody

Catalogue Numbers:BS4784-50, BS4784-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99704

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC, IF

All Applications: IHC: 1:50~1:200
IF: 1:50~1:200

Background: Dok-1 associates with the Ras GTPase-activating protein (Ras GAP) upon tyrosine phosphorylation. Evidence suggests that Dok-1 (also designated p62dok) is a substrate of the constitutive tyrosine kinase activity of p210 Bcr-Abl, a fusion protein caused by the t(9;22) translocation and associated with chronic myelogenous leukemia. Dok-1, as well as the tyrosine kinase substrates IRS-1 and Cas, are members of a class of “docking” proteins which contain multiple tyrosine residues and putative SH2 binding sites. Dok-1 is suspected to be the substrate phosphorylated in response to stimulation by a number of growth factors, including PDGF, VEGF, insulin and IGF.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Bioworld Molecular Weight: ~ 62 kDa

Specificity: p-Dok-1 (Y362) polyclonal antibody detects endogenous levels of Dok-1 protein only when phosphorylated at Tyr362.

Note: For research use only, not for use in diagnostic procedure.
Alternative Name: Docking protein 1 62kD; Docking protein 1; DOK 1; DOK1; DOK1; Downstream of tyrosine kinase 1; p62(dok); P62DOK; pp62

Immunogen: Synthetic phosphopeptide derived from human Dok-1 around the phosphorylation site of Tyrosine 362.

Conjugate: Unconjugated

Modification: Phosphorylation